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| Ninhaus-Silveira,Alexandre; Foresti,Fausto; Tabata,Yara Aiko; Rigolino,Marcos Guilherme; Veríssimo-Silveira,Rosicleire. |
| Cryopreservation of semen from sex-reversed females of rainbow trout aims at rationalizing the production of stocks composed by 100% females. Semen from normal males (M) and two types of genotypic females (R and G), sex-reversed by the oral administration of 17alpha-methyltestosterone, were used. R was obtained by the fertilization of normal eggs with semen of sex-reversed females while G via gynogenetic reproduction. Semen was diluted in an extender solution (glucose 5,4 g, egg yolk 10 ml, dimetil sulfoxide 10 ml, water 80 ml) at 1:3 ratio (semen/extender), stored in straws of 0.5 ml and freezed in a dry container Cryopac CP-65, at -180ºC. Thawing was performed with water at 70ºC for 3 seconds. There were no significant fertilization rate differences... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Oncorhynchus mykiss; Cryopreservation; Sex reversal; Gynogenesis; Semen. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000100009 |
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| Costa,Raphael S.; Souza,Fabrício M. S. de; Senhorini,José A.; Veríssimo-Silveira,Rosicleire; Ninhaus-Silveira,Alexandre. |
| ABSTRACT This study evaluated the effect of the cryoprotectants and the low temperatures on the embryonic development of Prochilodus lineatus, describing their main morphological alterations. On chilling sensitivity test, the survival rates at the twenty somites stage (20S) were 53.6% at 0ºC, and 100% in 5ºC. To test toxicity, the embryos were exposed to a graded series of 1,2-Propanediol (PROP), dimethyl sulfoxide (Me2SO4) and glycerol (GLY), terminating in a solution of high osmolarity. There was no significant difference in the embryos survival of toxicity test between series of PROP and Me2SO4 in the 6S and 20S. In the cooling protocols, were evaluated the effects of low temperature associated with cryoprotectants. At 5ºC, PROP showed survival rates... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cooling; Cryopreservation; Injuries; Morphology; Toxicity. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252017000300201 |
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| Ninhaus-Silveira,Alexandre; Foresti,Fausto; Veríssimo-Silveira,Rosicleire; Senhorini,José Augusto. |
| Aiming to improve fish reproduction techniques, the characterization and cryopreservation of semen of Brycon cephalus were performed. The seminal characteristics observed were: an almost transparent, milky semen with a mean volume of 4mL, and sperm concentration of 9.617±1.630 x 10(6) spermatozoa/mm³. Spermatozoa (length = 31.288 ±4.47µm) were of the aquasperm type and displayed a small, round head (length = 1.727 ±0.18 µm; width = 1.752 ±0.17µm) without acrosomal vesicle, nucleus with highly condensed chromatin forming coarse clots and centriolar complex located in the nuclear fossa; a midpiece (length = 2.561±0.44µm), narrowed rearward, with a cytoplasmic canal; and a flagellum (length = 29.521 ±4.37µm). Fertilization tests with thawed semen demonstrated... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Semen characteristics; Cryopreservation; Brycon cephalus; Ultrastructure; Fish. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000500015 |
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| Carneiro-Leite,Laicia; Bashiyo-Silva,Cristiane; Oliveira,Yasmim Alves Araújo; Borges,Laís Pedroso; Sanchez,Malbelys Padilla; Silva,Luciane Gomes da; Lobato,Stella Indira Rocha; Rodrigues-Lisoni,Flávia Cristina; Veríssimo-Silveira,Rosicleire; Ninhaus-Silveira,Alexandre. |
| Abstract This study aimed to determine the semen characteristics of Astyanax lacustris after hormonal induction and to evaluate the sensitivity of the species sperm to cryoprotective solutions based on the cryoprotectants dimethyl sulfoxide and methyl glycol. Volume, color, sperm concentration, total motility and aspects of sperm movement were analyzed using “Integrated Semen Analysis System”. Three different extenders were tested: A) glucose 5%+egg yolk 10%, B) BTS®5% and C) glucose 5% and two permeable cryoprotectants: dimethyl sulfoxide (Me2SO) and methyl glycol (MTG). Fresh A. lacustris semen presented total motility of 76.6±11.2%, motility duration of 33.0±2.2s, sperm concentration of 7.22±3.2×109sptz/mL and seminal osmolality of 219±0.03mOsm/kg-1.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Characiformes; DNA integrity; Freezing; Semen quality; Yellow-tailed Lambari. |
| Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252020000300214 |
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| Buzollo,Hellen; Veríssimo-Silveira,Rosicleire; Oliveira-Almeida,Isângela R.; Alexandre,Juliana S.; Okuda,Hélio T.; Ninhaus-Silveira,Alexandre. |
| The fish embryonic development comprises the events between the egg fertilization up to larvae hatching, being useful for the identification of viable eggs in productivity and survival studies as well as in raising experiments of several species. The goal of the present study was to characterize the embryonic development of Pimelodus maculatus (Siluriformes; Pimelodidae). The embryogenesis was typical of teleosteans, but with differences in relation to other species such as duration of development, type of blastocoel, moment of somite segmentation among others. Six stages of embryonic development were defined: zygote, cleavage, blastula, gastrula, organogenesis (divided in phases: early segmentation and late segmentation) and hatching with a period of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Embryonic development; Induced spawning; Morphology; Yolk syncyctial layer. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252011000300013 |
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